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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MUC5B Antibody (aa4698‑4707) LS‑C154788
Antibody
MUC5B antibody LS-C154788 is an unconjugated goat polyclonal antibody to mouse MUC5B. Validated for Peptide-ELISA.
Catalog
Size
Price
LS-C154788-100
100 µg (0.5 mg/ml)
Unavailable

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Western blot of recombinant MUC5B.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: IHC, Western blot

Product Description

MUC5B antibody LS-C154788 is an unconjugated goat polyclonal antibody to mouse MUC5B. Validated for Peptide-ELISA.
About MUC5B
MUC5B is a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. Q9HC84 NM_002458 NP_002449.2

MUC5B Antibody, MUC-5B Antibody, MUC9 Antibody, Mucin-5B Antibody, Sublingual gland mucin Antibody, MG1 Antibody, Cervical mucin Antibody, Cervical mucin MUC5B Antibody, MUC5 Antibody


Specifications

Target
Mouse MUC5B
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Immunogen
MUC5B antibody was raised against synthetic peptide CEKEDSSTCQ from an internal region of mouse MUC5B (NP_083077.2). Percent identity by BLAST analysis: Mouse (100%); Hamster (80%).
Blocking Peptide
LS-E27468 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C154788. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa4698-4707
Specificity
Mouse MUC5B.
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments gave bands at approx 100kD and 55kD in Mouse Lung lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 515kD according to Mouse NP_083077.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/23/2018

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