(tested or 100% immunogen sequence identity)
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
MUC5B antibody was raised against synthetic peptide CEKEDSSTCQ from an internal region of mouse MUC5B (NP_083077.2). Percent identity by BLAST analysis: Mouse (100%); Hamster (80%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C154788. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments gave bands at approx 100kD and 55kD in Mouse Lung lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 515kD according to Mouse NP_083077.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.