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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2434-50 | 50 µg (1 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Human MUC5AC Antibody (clone CLH2, IHC) LS‑B2434
IHC‑plus™ Monoclonal Mouse anti‑Human MUC5AC Antibody (clone CLH2, IHC) LS‑B2434
Note: This antibody replaces LS-C16663
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
MUC5AC antibody LS-B2434 is an unconjugated mouse monoclonal antibody to human MUC5AC. Validated for ELISA, ICC, IHC and IP. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human MUC5AC
Synonyms
MUC5AC | Gastric mucin | Major airway glycoprotein | Muc5/5ac | Mucin-5AC | LeB | Lewis B blood group antigen | Tracheobronchial mucin | TBM | MUC-5AC | MUC5
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
CLH2
Purification
Purified
Modifications
Unmodified
Immunogen
Synthetic peptide of the MUC5AC tandem repeat.
Specificity
Recognizes the MUC5AC tandem repeat of human mucin. The predominant staining pattern in normal tissues is perinuclear. Antibody reactivity has been observed in foveolar and mucopeptic neck cells of normal gastric mucosa.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen (1:500 - 1:1000)
- ICC
- Immunoprecipitation
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2434 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2434 was determined to be 10 ug/ml. Positive control: Normal human epithelial tissues: bronchus, endocervix, stomach and gallbladder.
Presentation
PBS, pH 7.6, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MUC5AC
Validation
Anti-MUC5AC antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-MUC5AC antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ MUC5AC Antibody (clone CLH2) for IHC, ICC, IP, ELISA LS-B2434 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Analysis of mucosal mucins separated by SDS-urea agarose polyacrylamide composite gel electrophoresis. Samah M A Issa, Benjamin L Schulz, Nicolle H Packer, Niclas G Karlsson. Electrophoresis. 2011 December;32:3554-63.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
