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Anti-MUC5AC Antibody (clone 1-13M1) IHC-plus™ LS-B5563

Note: This antibody replaces LS-C134848

Catalog	Size	Price
LS-B5563-50	50 µg (0.1 mg/ml)	Unavailable

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Anti-MUC5AC Antibody (clone 45M1, Biotin) LS-C87775

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Formalin-fixed, paraffin-embedded rat stomach stained with peroxidase-conjugate and DAB chromogen. Note cytoplasmic and surface staining of mucous cells. This image was taken for the unmodified form of this product. Other forms have not been tested.

Anti-MUC5AC Antibody (clone 1-13M1) IHC-plus™ LS-B5563

Antibody:	Mouse Monoclonal [clone 1-13M1] (IgG1) to Human MUC5AC
Reactivity:	Human
Application:	IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Format:	Unconjugated

Anti-MUC5AC antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5563 dilution 1:100.

Anti-MUC5AC Antibody (clone 2-12M1) LS-C146614

Antibody:	Mouse Monoclonal [clone 2-12M1] (IgG1) to Human MUC5AC
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Anti-MUC5AC Antibody (aa4944-5030, Biotin) LS-C300969

Antibody:	Rabbit Polyclonal (IgG) to Human MUC5AC
Reactivity:	Human
Application:	Western blot
Format:	Biotin Conjugated

Western blot of recombinant MUC5AC. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Antibody:	Mouse Monoclonal [clone 1-13M1] (IgG1) to Human MUC5AC
Reactivity:	Human
Application:	IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Format:	Unconjugated

Details

Target:	Human MUC5AC
Host:	Mouse
Reactivity:	Human (tested or 100% immunogen sequence identity)
Clonality:	IgG1 Monoclonal [1-13M1]
Conjugations:	Unconjugated
Purification:	Purified
Modifications:	Unmodified

Applications

Approved:

IHC
IHC - Paraffin (1:100)
IHC - Frozen
Western blot
ELISA

Specificity and Use

Immunogen:	MUC5AC antibody was raised against mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).
Specificity:	1-13M1 reacts with peptide core of gastric mucin (MUC5AC).
Usage:	Immunohistochemistry on cultured cells. Immunochemistry on frozen ethanol fixed section. Immunochemistry on paraffin section: a protease pretreatment is recommended. monoclonal antibody cross-reacts with mucin of different animal species after period date pretreatment. Western Blot: strongly positive without mercaptoethanol pretreatment of mucin solution. ELISA or IRMA in combination with the 9-13M1 monoclonal antibody or the mixture of anti-M1 MoAbs.

Packaging

Presentation:	PBS, 0.05% sodium azide
Storage:	Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Restrictions:	For research use only.

About MUC5AC

Uniprot: P98088 NCBI: AJ001403 CAA04738.1

Synonyms: MUC5AC Antibody, Gastric mucin Antibody, Major airway glycoprotein Antibody, Muc5/5ac Antibody, Mucin-5AC Antibody, LeB Antibody, Lewis B blood group antigen Antibody, Tracheobronchial mucin Antibody, TBM Antibody, MUC-5AC Antibody, MUC5 Antibody

Description: MUC5AC has been isolated from airway secretions. It is found in the protective film of mucus coating the mucosal surfaces of the respiratory tract and the normal gastrointestinal tissues. Mucins are also expressed at low levels in human fetal gut. MUC5AC is involved in epithelial cell proliferation and differentiation in adult airway mucosa and can be used as a marker for hypersecretory disorders. MUC5AC has also been observed in various tumors.

(More About MUC5AC)

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Immunohistochemistry

Anti-MUC5AC antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5563 dilution 1:100.

Requested From:
Date Requested: 4/23/2018

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