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Anti-MtTFA / TFAM Antibody (aa216-246) LS-C161815

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MtTFA / TFAM Antibodies

Anti-MtTFA / TFAM Antibody (aa169-218) LS-C30495
Rabbit Polyclonal (IgG) to Mouse MtTFA / TFAM
Mouse, Rat, Bovine, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MtTFA / TFAM Antibody (aa75-124) IHC-plus™ LS-B1571
Rabbit Polyclonal (IgG) to Human MtTFA / TFAM
Human, Mouse, Dog, Guinea pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MtTFA / TFAM Antibody (clone 18G102B2E11) IHC-plus™ LS-B9989
Mouse Monoclonal [clone 18G102B2E11] (IgG2b,k) to Human MtTFA / TFAM
Human, Rat
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human MtTFA / TFAM
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human MtTFA / TFAM
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

MtTFA / TFAM antibody was raised against kLH-conjugated synthetic peptide between amino acids 216-246 from C-terminal region of human TFAM.
LS-E144 - Lyophilized - 100 µg - $145.00
aa216-246
Human MtTFA / TFAM

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About MtTFA / TFAM

Q00059 NM_003201 NP_003192.1

TFAM Antibody, MTTF1 Antibody, TCF-6 Antibody, TCF6 Antibody, TCF6L1 Antibody, Transcription factor 6-like 2 Antibody, Transcription factor 6-like 3 Antibody, TCF6L2 Antibody, TCF6L3 Antibody, Transcription factor 6 Antibody, Transcription factor 6-like 1 Antibody, MTTFA Antibody

Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes.

Immunohistochemistry

Immunohistochemistry
TFAM antibody immunohistochemistry of formalin-fixed and paraffin-embedded human testis carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of NCI-H460 cell stained with TFAM Antibody. NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAM primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TFAM immunoreactivity is localized to mitochondrion significantly.

Western blot

Western blot
TFAM Antibody western blot of HeLa,Jurkat,K562,MCF-7 cell line lysates (35 ug/lane). The TFAM antibody detected the TFAM protein (arrow).

Western blot

Western blot
Western blot of lysate from 293T cell line, using TFAM Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Flow Cytometry

Flow Cytometry
TFAM Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

Requested From: United States
Date Requested: 12/11/2016

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