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Catalog Number Size Price
LS-C161815-400 400 µl $393 
MtTFA / TFAM Antibody - TFAM antibody immunohistochemistry of formalin-fixed and paraffin-embedded human testis carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
MtTFA / TFAM Antibody - Fluorescent confocal image of NCI-H460 cell stained with TFAM Antibody. NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAM primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TFAM immunoreactivity is localized to mitochondrion significantly.
MtTFA / TFAM Antibody - TFAM Antibody western blot of HeLa,Jurkat,K562,MCF-7 cell line lysates (35 ug/lane). The TFAM antibody detected the TFAM protein (arrow).
MtTFA / TFAM Antibody - Western blot of lysate from 293T cell line, using TFAM Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
MtTFA / TFAM Antibody - TFAM Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MtTFA / TFAM Antibody - TFAM antibody immunohistochemistry of formalin-fixed and paraffin-embedded human testis carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
MtTFA / TFAM Antibody - Fluorescent confocal image of NCI-H460 cell stained with TFAM Antibody. NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAM primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TFAM immunoreactivity is localized to mitochondrion significantly.
MtTFA / TFAM Antibody - TFAM Antibody western blot of HeLa,Jurkat,K562,MCF-7 cell line lysates (35 ug/lane). The TFAM antibody detected the TFAM protein (arrow).
MtTFA / TFAM Antibody - Western blot of lysate from 293T cell line, using TFAM Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
MtTFA / TFAM Antibody - TFAM Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human MtTFA / TFAM Antibody (aa216‑246, IHC, IF, WB) LS‑C161815

Polyclonal Rabbit anti‑Human MtTFA / TFAM Antibody (aa216‑246, IHC, IF, WB) LS‑C161815

Antibody:
MtTFA / TFAM Rabbit anti-Human Polyclonal (aa216-246) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C161815-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MtTFA / TFAM Rabbit anti-Human Polyclonal (aa216-246) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TFAM antibody LS-C161815 is an unconjugated rabbit polyclonal antibody to human TFAM (MtTFA) (aa216-246). Validated for Flow, IF, IHC and WB.
Target
Human MtTFA / TFAM
Synonyms
TFAM | MTTF1 | TCF-6 | TCF6 | TCF6L1 | Transcription factor 6-like 2 | Transcription factor 6-like 3 | TCF6L2 | TCF6L3 | Transcription factor 6 | Transcription factor 6-like 1 | MTTFA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa216-246
Specificity
This TFAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the C-terminal region of human TFAM.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MtTFA / TFAM
Q00059 NM_003201 NP_003192.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024