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Anti-MTRPOL / POLRMT Antibody (aa1205-1218) LS-C205050

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $275
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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Anti-MTRPOL / POLRMT Antibody (clone 3D9) LS-C197949
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Anti-MTRPOL / POLRMT Antibody (aa933-982) LS-C201550
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Anti-MTRPOL / POLRMT Antibody (aa933-982, Biotin) LS-C451447
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100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human MTRPOL / POLRMT
Human, Monkey
ELISA
Unconjugated

Details

Human MTRPOL / POLRMT
Goat
Human, Monkey (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

ELISA (1:128000)

Specificity and Use

MTRPOL / POLRMT antibody was raised against synthetic peptide C-KETLQAVPKPGAFD from the C-terminus of human MTRPOL / POLRMT (NP_005026.3). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey (100%); Gibbon (93%); Mouse (64%).
aa1205-1218
Human MTRPOL / POLRMT
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave bands at approx 90kD and 35kD in Human HepG2 lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 139kD according to NP_005026.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Packaging

Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About MTRPOL / POLRMT

O00411 NM_005035 NP_005026.3

POLRMT Antibody, APOLMT Antibody, H-mtRPOL Antibody, MTRPOL Antibody, MTRNAP Antibody

MTRPOL / POLRMT is a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.

Requested From: United States
Date Requested: 12/3/2016

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