Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Protein A purified
- Western blot (1:1000)
- Immunoprecipitation (1:100)
MTOR antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser2448 of human mTOR.
Detects endogenous levels of human mTOR only when phosphorylated at Ser2448. Species cross-reactivity: Mouse and rat.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:100.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.