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MTNR1B / MT2 Antibody (aa334‑348) IHC‑plus™ LS‑B7370
Note: This antibody replaces LS-C112709
MT2 antibody LS-B7370 is an unconjugated goat polyclonal antibody to human MT2 (MTNR1B). Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
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Technical Help
Review This ProductPopular MTNR1B / MT2 Products
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA
Product Description
MT2 antibody LS-B7370 is an unconjugated goat polyclonal antibody to human MT2 (MTNR1B). Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
About MTNR1B / MT2
MTNR1B / MT2 is one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This gene product is an integral membrane protein that is a G-protein coupled, 7-transmembrane receptor. It is found primarily in the retina and brain although this detection requires RT-PCR. It is thought to participate in light-dependent functions in the retina and may be involved in the neurobiological effects of melatonin.
P49286
NM_005959
NP_005950.1
MTNR1B Antibody, MEL-1B-R Antibody, Mel-1b melatonin receptor Antibody, Melatonin receptor 1B Antibody, Melatonin receptor MEL1B Antibody, Mt2r Antibody, MT2 melatonin receptor Antibody, MtT2 receptor Antibody, Melatonin receptor type 1B Antibody, MT2 Antibody, FGQTL2 Antibody, Mel1b receptor Antibody
Specifications
Target
Human MTNR1B / MT2
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
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Immunogen
MTNR1B / MT2 antibody was raised against synthetic peptide C-QDASKGSHAEGLQSP from the C-terminus of human MTNR1B (NP_005950.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Monkey (87%); Elephant (80%).
Blocking Peptide
LS-E26943 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B7370. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-B7370. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa334-348
Specificity
Human MTNR1B.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave approx .48kDa and 37kDa bands in Human Brain (Cerebellum) lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_005950.1. The 48kDa and 37kDa bands were successfully blocked by incubation with the immunizing peptide.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Immunohistochemistry
Anti-MTNR1B antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7370 concentration 3.75 ug/ml.
Immunohistochemistry
Anti-MTNR1B antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7370 concentration 3.75 ug/ml.
Immunohistochemistry
Anti-MTNR1B antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7370 concentration 3.75 ug/ml.
Immunohistochemistry
Anti-MTNR1B antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7370 concentration 3.75 ug/ml.
Requested From:
Date Requested: 6/23/2018
Date Requested: 6/23/2018
