Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Myotubularin (MTM1), a myotubularin-related phosphatase, has been shown to contain the consensus active site of dual specificity protein phosphatases (DSPs) and to share inhibition and inactivation characteristics with other DSPs in vitro and in vivo. MTM1 also has been shown to possess a SET-interacting domain (SID) which is unique to myotubularin-related proteins.
Immunoperoxidase of monoclonal antibody to MTM1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
Western blot of MTM1 expression in transfected 293T cell line by MTM1 monoclonal antibody (M01), clone 1C10.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged MTM1 is approximately 1 ng/ml as a capture antibody.
Western blot of MTM1 over-expressed 293 cell line, cotransfected with MTM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MTM1 monoclonal antibody, clone 1C10. GAPDH ( 36.1 kD ) used as specificity a.