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Anti-MTHFR Antibody (aa369-383) LS-C154913

Catalog Size Price
LS-C154913-100 100 µg (0.5 mg/ml) Unavailable

Related Products

35 MTHFR Antibodies

Most Popular MTHFR Antibodies

Anti-MTHFR Antibody (aa369-383) LS-C154913
Goat Polyclonal to Human MTHFR
Human, Monkey, Bovine, Dog, Rabbit
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
MTHFR antibody (0.3 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-MTHFR Antibody (aa266-292) LS-C157974
Rabbit Polyclonal to Human MTHFR
Human
Western blot
Unconjugated
MTHFR Antibody western blot of CEM cell line lysates (35 ug/lane). The MTHFR antibody detected the MTHFR protein (arrow).
Anti-MTHFR Antibody (aa685-697) IHC-plus™ LS-B9203
Goat Polyclonal to Human MTHFR
Human
IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-MTHFR antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MTHFR Antibody (Internal) LS-C352540
Rabbit Polyclonal to Human MTHFR
Human, Monkey, Mouse
Western blot
Unconjugated
Western blot analysis of MTHFR expression in HEK293 (A); Jurkat (B); A549 (C) whole cell lysates.
Anti-MTHFR Antibody (aa1-325, Biotin) LS-C689005
Rabbit Polyclonal (IgG) to Human MTHFR
Human
IHC, Western blot
Biotin Conjugated
Western blot of MTHFR antibody.

100% Guaranteed
Goat Polyclonal to Human MTHFR
Human, Monkey, Bovine, Dog, Rabbit
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated

Details

Human MTHFR
Goat
Human, Monkey, Bovine, Dog, Rabbit (tested or 100% immunogen sequence identity)
Mouse, Rat, Bat, Hamster, Horse, Chicken, Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)

Specificity and Use

MTHFR antibody was raised against synthetic peptide C-SRPKSYIYRTQEWDE from an internal region of human MTHFR (NP_005948.3). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Rabbit, Porcine, Medaka (100%); Mouse, Rat, Hamster, Bat, Horse, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus (93%); Guinea pig, Stickleback, Pufferfish, Zebrafish (87%); Seq squirt, Sea anemone, Water flea (80%).
LS-E27776 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C154913. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa369-383
Human MTHFR.
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 70kD band observed in lysates of cell line NIH3T3 (calculated MW of 74.6kD according to Human NP_005948.3 and Mouse NP_034970.2). Recommended concentration: 0.3-1 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About MTHFR

P42898 NM_005957 NP_005948.3

MTHFR Antibody

MTHFR catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.

Western blot

MTHFR antibody (0.3 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
MTHFR antibody (0.3 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 5/26/2018

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