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Anti-MTHFD1 Antibody (aa863-912) IHC-plus™ LS-B5794

Note: This antibody replaces LS-C111089

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MTHFD1 Antibodies

Anti-MTHFD1 Antibody (aa137-150) IHC-plus™ LS-B4110
Goat Polyclonal to Human MTHFD1
Human, Monkey, Mouse, Bat, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MTHFD1 Antibody (aa74-123) IHC-plus™ LS-B5793
Rabbit Polyclonal (IgG) to Human MTHFD1
Human, Monkey, Mouse, Rat, Pig, Rabbit, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MTHFD1 Antibody (aa535-562) LS-C164661
Rabbit Polyclonal to Human MTHFD1
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human MTHFD1
Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human MTHFD1
Rabbit
Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Bovine, Hamster, Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

MTHFD1 antibody was raised against synthetic peptide located between aa863-912 of human MTHFD1 (P11586, NP_005947). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Dog, Bat, Rabbit, Horse, Pig (100%); Hamster, Bovine, Xenopus (92%); Elephant, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Slime mold (85%).
LS-E16833 - Lyophilized - 100 µg - $145.00
aa863-912
Human MTHFD1
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MTHFD1

P11586 NM_005956 NP_005947.3

MTHFD1 Antibody, C1-THF synthase Antibody, MTHFC Antibody, MTHFD Antibody

MTHFD1 encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses.

Immunohistochemistry

Immunohistochemistry
Anti-MTHFD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5794 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
MTHFD1 antibody LS-B5794 Western blot of OVCAR-3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/8/2016

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