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Catalog Number Size Price
LS-C19034-100 100 µl $574 
MTBP / Mdm2-Binding Protein Antibody - Western blot using anti-MTBP antibody shows detection of a band ~110 kDa corresponding to human MTBP (arrowhead). Lanes represent human 293 cell lysates with (+) and without (-) transfection with a full-length human expression MTBP construct. The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP. Proteins were separated by SDS-PAGE and transferred onto PDVF membrane. After blocking, the membrane was probed with the primary antibody diluted to 1:500 for 2h at room temperature followed by detection using a Lumi-LightPlus Western Blotting Kit (Roche).

Monoclonal Mouse anti‑Human MTBP / Mdm2‑Binding Protein Antibody (clone PHL‑1, aa667‑812, WB) LS‑C19034

Monoclonal Mouse anti‑Human MTBP / Mdm2‑Binding Protein Antibody (clone PHL‑1, aa667‑812, WB) LS‑C19034

Antibody:
MTBP / Mdm2-Binding Protein Mouse anti-Human Monoclonal (aa667-812) (PHL-1) Antibody
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified
Price
Catalog Number
$574
LS-C19034-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MTBP / Mdm2-Binding Protein Mouse anti-Human Monoclonal (aa667-812) (PHL-1) Antibody
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified

Specifications

Description
Mdm2-Binding Protein antibody LS-C19034 is an unconjugated mouse monoclonal antibody to Mdm2-Binding Protein (MTBP) (aa667-812) from human. It is reactive with human and chimpanzee. Validated for ELISA and WB.
Target
Human MTBP / Mdm2-Binding Protein
Synonyms
MTBP | Mdm2-binding protein | MDM2BP | HMTBP
Host
Mouse
Reactivity
Human, Chimpanzee (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee (at least 90% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
PHL-1
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant amino-terminal HIS6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein.
Epitope
aa667-812
Specificity
This antibody is directed against human MTBP protein. The product was produced from clarified mouse ascetic fluid. A BLAST analysis was used to suggest reactivity with this protein from human (100% homology) and chimpanzee. Cross reactivity with MTBP protein from mouse (77% homology) and chicken (79% homology) is unlikely due to the relatively low number of positive residues and high number of gaps within this sequence. Cross reactivity with MTBP homologues from other sources has not been determined.
Applications
  • Western blot (1:200 - 1:2000)
  • ELISA (1:2000 - 1:20000)
Usage
This antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band - 110 kD in size corresponding to MTBP by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MTBP / Mdm2-Binding Protein
Q96DY7 NM_022045 NP_071328.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024