Mycobacterium tuberculosis MTB / M. tuberculosis
(tested or 100% immunogen sequence identity)
Protein A/G purified
- Western blot
- ELISA (1:3160)
PPD from Mycobacterium tuberculosis (Tuberculin PPD, RT23, Statens Serum Institut). Percent identity by BLAST analysis: M. tuberculosis (100%).
Recognizes a 10kD band from short term culture of M. tuberculosis identified as the protein ESAT-6 (Rv3875) in Western Blot. Binds to an epitope consisting of the residues EQQWNFAGIEAAA at positions 3-15. Species cross-reactivity: Mycobacterium kansasii, Mycobacterium szulgai, and Mycobacterium marinum. Reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis.
Suitable for use in ELISA and Western Blot. ELISA: 1:3160. Optimal dilutions. to be determined by the researcher. Hybridoma: X63-Ag8.653. Female CF1 x Balb/c mice immunized i. p. Culture Medium: RPMI 1640 with 10% fetal calf serum.
PBS, pH 7.2, 15 mM sodium azide, 40% glycerol.
For research use only.
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