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Anti-MSLN / Mesothelin Antibody (Extracellular Domain, clone MB-G10) LS-C154007


Wt. Vol. Conc. Price
100 µg - 1 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular MSLN / Mesothelin Antibodies

Anti-MSLN / Mesothelin Antibody (clone 295D) LS-C179303
Rat Monoclonal [clone 295D] (IgG2a,k) to Mouse MSLN / Mesothelin
Flow Cytometry
Flow Cytometry Image
Anti-MSLN / Mesothelin Antibody (clone 295D, PE) LS-C179304
Rat Monoclonal [clone 295D] (IgG2a,k) to Mouse MSLN / Mesothelin
Flow Cytometry
PE Conjugated
Flow Cytometry Image
Anti-MSLN / Mesothelin Antibody (clone B35) LS-C179484
Rat Monoclonal [clone B35] (IgG2a) to Mouse MSLN / Mesothelin
Flow Cytometry
Flow Cytometry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone MB-G10] (IgG) to Human MSLN / Mesothelin
IHC, Western blot, ELISA


Human MSLN / Mesothelin
Human (tested or 100% immunogen sequence identity)
IgG Monoclonal [MB-G10]
Protein A purified


  • IHC (1:100)
  • Western blot (1:1000)

Specificity and Use

MSLN / Mesothelin antibody was raised against this antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to the extracellular domain of human mesothelin.
Extracellular Domain
Cross reactivity with homologues from other sources has not been tested.
This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kD in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Onda M, Willingham M, Nagata S, Bera TK, Beers R, Ho M, Hassan R, Kreitman RJ, Pastan I. Clinical cancer research : an official journal of the American Association for Cancer Research. 2005 11:5840-6. [PubMed:16115924]

About MSLN / Mesothelin

Q13421 NM_013404 NP_037536.2

MSLN Antibody, CAK1 antigen Antibody, MPF Antibody, SMRP Antibody, Mesothelin Antibody, CAK1 Antibody

This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. Mesothelin is a cell surface differentiation antigen found in mesothelial cells, mesotheliomas and ovarian cancers. Although the function of mesothelin is unknown, it may play a role in cellular adhesion. Three transcript variants have been described.


Anti-Mesothelin Antibody - Immunohistochemistry. Immunohistochemistry using anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission fromClin. Cancer Res. 11(16):5840-6.

Western blot

Western blot
Anti-Mesothelin Antibodies - Western Blot. Western blotting using anti-mesothelin antibodies to detect mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5). Lane 6 contains 50 ng of CDC25-Fc. Proteins were separated on 4-20% gradient gel by SDS-PAGE followed by transfer to PVDF membrane. Primary antibody was used at 1 ug/ml followed by reaction with ALP goat anti-mouse IgG and BCIP/NBT substrate. Reprinted with permission from Clin. Cancer Res. 11(16):5840-6.

Requested From: United States
Date Requested: 10/21/2016

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