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Anti-MSH5 Antibody (aa508-519) IHC-plus™ LS-B6469

Note: This antibody replaces LS-C87238
Catalog Size Price
LS-B6469-50 50 µg (0.5 mg/ml) Unavailable

Related Products

25 MSH5 Antibodies

Most Popular MSH5 Antibodies

Anti-MSH5 Antibody (aa508-519) IHC-plus™ LS-B6469
Goat Polyclonal to Human MSH5
Human, Monkey, Mouse, Chicken, Xenopus
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MSH5 Antibody (aa525-552) LS-C164648
Rabbit Polyclonal to Human MSH5
Human
Western blot
Unconjugated
Western blot Image
Anti-MSH5 Antibody (clone 1C11) LS-C197705
Mouse Monoclonal [clone 1C11] (IgG2a,k) to Human MSH5
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence Image
Anti-MSH5 Antibody LS-C400892
Rabbit Polyclonal (IgG) to Human MSH5
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MSH5 Antibody LS-C404146
Rabbit Polyclonal (IgG) to Human MSH5
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Goat Polyclonal to Human MSH5
Human, Monkey, Mouse, Chicken, Xenopus
IHC - Paraffin, ELISA
Unconjugated

Details

Human MSH5
Goat
Human, Monkey, Mouse, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Rat, Bovine, Dog, Horse, Pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 - 3.75 µg/ml)
  • ELISA (1:64000)

Specificity and Use

MSH5 antibody was raised against synthetic peptide GDLHCEIRDQET from an internal region of human MSH5 (NP_079535.3 ; NP_751897.1; NP_002432.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Mouse, Chicken, Xenopus (100%); Rat, Panda, Bovine, Dog, Horse, Pig (92%); Marmoset (83%).
LS-E26618 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6469. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa508-519
Human MSH5. This antibody is expected to recognise three reported isoforms ( NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein.
Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 38kD band in Human Heart, Tonsil and Thymus lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9kD according to the smallest reported isoform NP_002432.1. The 38kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About MSH5

O43196 NM_002441 NP_002432.1

MSH5 Antibody, MutS homolog 5 (E. coli) Antibody, HMSH5 Antibody, MutS protein homolog 5 Antibody, NG23 Antibody, G7 Antibody, MutS (E. coli) homolog 5 Antibody, MUTSH5 Antibody

MSH5 is a member of the mutS family of proteins that are involved in DNA mismatch repair and meiotic recombination. This protein is similar to a Saccharomyces cerevisiae protein that participates in segregation fidelity and crossing-over events during meiosis. This protein plays a role in promoting ionizing radiation-induced apoptosis. This protein forms hetero-oligomers with another member of this family, mutS homolog 4.

Immunohistochemistry

Immunohistochemistry
Anti-MSH5 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-MSH5 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.

Requested From: 
Date Requested: 6/24/2017

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