Human, Monkey, Mouse, Chicken, Xenopus
(tested or 100% immunogen sequence identity)
Rat, Bovine, Dog, Horse, Pig (at least 90% immunogen sequence identity)
- IHC - Paraffin (2.5 - 3.75 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
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MSH5 antibody was raised against synthetic peptide GDLHCEIRDQET from an internal region of human MSH5 (NP_079535.3 ; NP_751897.1; NP_002432.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Mouse, Chicken, Xenopus (100%); Rat, Panda, Bovine, Dog, Horse, Pig (92%); Marmoset (83%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6469. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human MSH5. This antibody is expected to recognise three reported isoforms ( NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein.
Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 38kD band in Human Heart, Tonsil and Thymus lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9kD according to the smallest reported isoform NP_002432.1. The 38kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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