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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MSH5 Antibody (aa508‑519) IHC‑plus™ LS‑B6469 Best Seller
Note: This antibody replaces LS-C87238
MSH5 antibody LS-B6469 is an unconjugated goat polyclonal antibody to MSH5 from human, mouse, chicken and other species. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6469-50
50 µg (0.5 mg/ml)
Unavailable

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Product Description

MSH5 antibody LS-B6469 is an unconjugated goat polyclonal antibody to MSH5 from human, mouse, chicken and other species. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
About MSH5
MSH5 is a member of the mutS family of proteins that are involved in DNA mismatch repair and meiotic recombination. This protein is similar to a Saccharomyces cerevisiae protein that participates in segregation fidelity and crossing-over events during meiosis. This protein plays a role in promoting ionizing radiation-induced apoptosis. This protein forms hetero-oligomers with another member of this family, mutS homolog 4. O43196 NM_002441 NP_002432.1

MSH5 Antibody, MutS homolog 5 (E. coli) Antibody, HMSH5 Antibody, MutS protein homolog 5 Antibody, NG23 Antibody, G7 Antibody, MutS (E. coli) homolog 5 Antibody, MUTSH5 Antibody


Specifications

Target
Human MSH5
Host
Goat
Reactivity
Human, Monkey, Mouse, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine, Dog, Horse, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 - 3.75 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MSH5 antibody was raised against synthetic peptide GDLHCEIRDQET from an internal region of human MSH5 (NP_079535.3 ; NP_751897.1; NP_002432.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Mouse, Chicken, Xenopus (100%); Rat, Panda, Bovine, Dog, Horse, Pig (92%); Marmoset (83%).
Blocking Peptide
LS-E26618 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6469. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa508-519
Specificity
Human MSH5. This antibody is expected to recognise three reported isoforms ( NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein.
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 38kD band in Human Heart, Tonsil and Thymus lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9kD according to the smallest reported isoform NP_002432.1. The 38kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-MSH5 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.
Anti-MSH5 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-MSH5 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.
Anti-MSH5 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-MSH5 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.
Anti-MSH5 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-MSH5 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.
Anti-MSH5 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-MSH5 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.
Anti-MSH5 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-MSH5 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.
Anti-MSH5 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-MSH5 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.
Anti-MSH5 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-MSH5 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.
Anti-MSH5 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6469 concentration 2.5 ug/ml.


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Date Requested: 6/20/2018

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