Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Protein A purified
IHC - Paraffin (2 µg/ml)
IHC - Frozen (2 µg/ml)
Western blot (0.2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
MSH2 antibody was raised against a carboxy-terminal fragment of the human MSH2 protein.
Recognizes human and mouse MSH2 proteins (~100kD).
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot (chemiluminescence): 0.2 ug/ml. Immunoprecipitation: 1 ug/sample. Immunohistochemistry (Frozen): 2 ug/ml. Immunohistochemistry (Paraffin): 2 ug/ml (staining of formalin fixed, paraffin embedded tissue requires Heat induced epitope retrieval (HIER) pretreatment). Positive control: Immunoprecipitation and Western Blot: HCT116 or SW480 cells.
PBS, pH 7.2, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA.