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Anti-MRPL12 Antibody (clone 3B12-1A3) LS-C133093


Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular MRPL12 Antibodies

Anti-MRPL12 Antibody (aa87-136) IHC-plus™ LS-B8280
Rabbit Polyclonal to Human MRPL12
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-MRPL12 Antibody (aa1-199, APC) LS-C279693
Mouse Monoclonal (IgG1) to Human MRPL12
IHC, ICC, Western blot, ELISA
APC Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3B12-1A3] (IgG1,k) to Human MRPL12
IHC - Paraffin, Immunofluorescence, Western blot, ELISA


Human MRPL12
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [3B12-1A3]
Protein A purified


  • IHC - Paraffin (3 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot

Specificity and Use

MRPL12 antibody was raised against mRPL12 (AAH02344, 1 a.a. ~ 199 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human MRPL12
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.


PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About MRPL12

P52815 NM_002949 NP_002940.2

MRPL12 Antibody, 5c5-2 Antibody, L12mt Antibody, MRP-L12 Antibody, MRP-L31/34 Antibody, MRPL7 Antibody, RPML12 Antibody, MRPL7/L12 Antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA.


Immunoperoxidase of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 3 ug/ml].


Immunofluorescence of monoclonal antibody to MRPL12 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
MRPL12 monoclonal antibody (M01), clone 3B12-1A3 Western blot of MRPL12 expression in COLO 320 HSR.

Western blot

Western blot
Western blot of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody (M01), clone 3B12-1A3.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged MRPL12 is approximately 0.03 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody, clone 3B12-1A3. GAPDH ( 36.1 kD ) used as specificity and loading control.

Requested From: United States
Date Requested: 10/28/2016

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