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IHCPlus™ MPO / Myeloperoxidase Antibody Polyclonal ELISA,IF,IHC,IP,WB LS‑B392

Catalog Number Size Price
LS-B392-50 50 µl (85 mg/ml) $425 
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot. Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kD and 15 kD polypeptides and chain combinations forming 68 kD and 106 kD proteins. The staining of the 68 kD band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot.  Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins.  The staining of the 68 kDa band is so intense that is over saturates the signal detection.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot. Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kD and 15 kD polypeptides and chain combinations forming 68 kD and 106 kD proteins. The staining of the 68 kD band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot.  Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins.  The staining of the 68 kDa band is so intense that is over saturates the signal detection.
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IHCPlus™ MPO / Myeloperoxidase Antibody Polyclonal ELISA,IF,IHC,IP,WB LS‑B392

Note: This antibody replaces LS-C19072
Antibody:
Rabbit Polyclonal to Human MPO / Myeloperoxidase
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B392-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human MPO / Myeloperoxidase
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Myeloperoxidase antibody LS-B392 is an unconjugated rabbit polyclonal antibody to human Myeloperoxidase (MPO ). Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MPO / Myeloperoxidase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Specificity
Myeloperoxidase [Human Leukocytes].
Usage
Immunohistochemistry: LS-B392 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B392 was determined to be 1:500.
Presentation
PBS, pH 7.2, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MPO / Myeloperoxidase
P05164 NM_000250 NP_000241.1

Validation

Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC Validated
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

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Requested From: United States
Date Requested: 9/23/2019