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Anti-MPO / Myeloperoxidase Antibody IHC-plus™ LS-B392

Note: This antibody replaces LS-C19072

Ordering

Wt. Vol. Conc. Price
- 50 µl 85 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MPO / Myeloperoxidase Antibodies

Anti-MPO / Myeloperoxidase Antibody (clone 9B12G7) IHC-plus™ LS-B3899
Mouse Monoclonal [clone 9B12G7] (IgG1) to Human MPO / Myeloperoxidase
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MPO / Myeloperoxidase Antibody IHC-plus™ LS-B4741
Rabbit Polyclonal (IgG) to Human MPO / Myeloperoxidase
Human, Mouse, Rat
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-MPO / Myeloperoxidase Antibody IHC-plus™ LS-B6699
Rabbit Polyclonal (IgG) to Human MPO / Myeloperoxidase
Human, Mouse, Rat
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human MPO / Myeloperoxidase
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human MPO / Myeloperoxidase
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • IHC - Paraffin (1:500)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA

Specificity and Use

Myeloperoxidase [Human Leukocytes].
Immunohistochemistry: LS-B392 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B392 was determined to be 1:500.

Packaging

PBS, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About MPO / Myeloperoxidase

P05164 NM_000250 NP_000241.1

MPO Antibody, Myeloperoxidase precursor Antibody, Myeloperoxidase Antibody

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains (12 kD) and 2 heavy chains (60 kD). This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.

Immunohistochemistry

Immunohistochemistry
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B392 dilution 1:500.

Western blot

Western blot
Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot. Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kD and 15 kD polypeptides and chain combinations forming 68 kD and 106 kD proteins. The staining of the 68 kD band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Western blot

Western blot
Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection.
 

Requested From: United States
Date Requested: 12/3/2016

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