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| Catalog Number | Size | Price |
|---|---|---|
| LS-B392-50 | 50 µl (85 mg/ml) | $775 |
![MPO / Myeloperoxidase Antibody - Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot. Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kD and 15 kD polypeptides and chain combinations forming 68 kD and 106 kD proteins. The staining of the 68 kD band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/ihc-plus-mpo-myeloperoxidase-antibody-ls-b392/62549_27856.jpg)
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IHC‑plus™ Polyclonal Rabbit anti‑Human MPO / Myeloperoxidase Antibody (IHC, WB) LS‑B392
IHC‑plus™ Polyclonal Rabbit anti‑Human MPO / Myeloperoxidase Antibody (IHC, WB) LS‑B392
Note: This antibody replaces LS-C19072
Antibody:
MPO / Myeloperoxidase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
MPO / Myeloperoxidase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Myeloperoxidase antibody LS-B392 is an unconjugated rabbit polyclonal antibody to human Myeloperoxidase (MPO). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MPO / Myeloperoxidase
Synonyms
MPO | Myeloperoxidase precursor | Myeloperoxidase
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
Myeloperoxidase [Human Leukocytes].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Myeloperoxidase [Human Leukocytes]. Cross reactivity against Myeloperoxidase from other tissues and species may occur but have not been specifically determined.
Applications
- IHC
- IHC - Paraffin (1:500)
- Western blot (1:5000)
- Immunoprecipitation (1:100)
- ELISA (1:200000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B392 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B392 was determined to be 1:500.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MPO / Myeloperoxidase
Validation
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
See More About...
LSBio Ratings
IHC-plus™ MPO / Myeloperoxidase Antibody for IHC, WB/Western, IP, ELISA LS-B392 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Mouse, Rat
Applications:
IHC, IHC - Frozen, Flow Cytometry
Species:
Mouse
Applications:
IHC, IHC - Frozen, Flow Cytometry
Reactivity:
Human
Range:
1.56-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
