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Anti-MOB4 / PHOCN Antibody (aa116-130) IHC-plus™ LS-B9537

Note: This antibody replaces LS-C139760

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml Unavailable

Most Popular MOB4 / PHOCN Antibodies

Anti-MOB4 / PHOCN Antibody (aa1-50) LS-C119342
Rabbit Polyclonal (IgG) to Human MOB4 / PHOCN
Human, Mouse, Rat
Western blot, ELISA
Unconjugated
Western blot Image
Anti-MOB4 / PHOCN Antibody (aa1-30) LS-C164261
Rabbit Polyclonal to Human MOB4 / PHOCN
Human
Western blot
Unconjugated
Western blot Image
Anti-MOB4 / PHOCN Antibody (aa10-59) LS-C200203
Rabbit Polyclonal (IgG) to Human MOB4 / PHOCN
Human, Mouse, Rat
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human MOB4 / PHOCN
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Chicken, Xenopus, Zebrafish, Drosophila, Insect
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human MOB4 / PHOCN
Goat
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Chicken, Xenopus, Zebrafish, Drosophila, Insect (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • ELISA (1:4000)

Specificity and Use

MOB4 / PHOCN antibody was raised against synthetic peptide C-NSNKYFPSRVSIKES from an internal region of human MOBKL3 (NP_056202.2; NP_955776.1; NP_001094289.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Opossum, Turkey, Chicken, Xenopus, Trout, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Sea anemone, Nematode, Beetle, Tick (100%); Catfish, Seq squirt, Eye worm, Ant, Water flea (93%); Blood fluke (80%).
LS-E27638 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9537. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa116-130
Human MOBKL3. This antibody is expected to recognize all three reported isoforms (NP_056202.2; NP_955776.1; NP_001094289.1).
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 26kD band observed in Human Brain (Frontal Cortex) and in Mouse and Rat Brain lysates (calculated MW of 26.0kD according to NP_056202.2). Recommended concentration: 0.3-1 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About MOB4 / PHOCN

Q9Y3A3 NM_015387 NP_056202.2

MOB4 Antibody, 2C4D Antibody, Class II mMOB1 Antibody, DKFZP564M112 Antibody, CGI-95 Antibody, MOB1 Antibody, MOB-like protein phocein Antibody, Mob1 homolog 3 Antibody, PREI3 Antibody, Preimplantation protein 3 Antibody, MOB3 Antibody, PHOCN Antibody, MOB family member 4, phocein Antibody, MOBKL3 Antibody, Phocein, Mob-like protein Antibody

MOB4 / PHOCN was identified based on its similarity with the mouse counterpart. Studies of the mouse counterpart suggest that the expression of this gene may be regulated during oocyte maturation and preimplantation following zygotic gene activation. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Naturally occurring read-through transcription occurs between this locus and the neighboring locus HSPE1.

Immunohistochemistry

Immunohistochemistry
Anti-MOBKL3 / MOB1 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9537 concentration 5 ug/ml.

Western blot

Western blot
MOBKL3 antibody (0.3 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 3/25/2017

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