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Antibodies > MOB1B / MOBKL1A > LS-C98948
 
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Catalog Number Size Price
LS-C98948-400 400 µl $393 
MOB1B / MOBKL1A Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
MOB1B / MOBKL1A Antibody - Fluorescent image of NIH/3T3 cell stained with MOB4A Antibody. NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MOB4A primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). MOB4A immunoreactivity is localized to Cytoplasm significantly.
MOB1B / MOBKL1A Antibody - MOB4A Antibody western blot of Daudi,K562 cell line lysates (35 ug/lane). The MOB4A antibody detected the MOB4A protein (arrow).
MOB1B / MOBKL1A Antibody - Western blot of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
MOB1B / MOBKL1A Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
MOB1B / MOBKL1A Antibody - Fluorescent image of NIH/3T3 cell stained with MOB4A Antibody. NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MOB4A primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). MOB4A immunoreactivity is localized to Cytoplasm significantly.
MOB1B / MOBKL1A Antibody - MOB4A Antibody western blot of Daudi,K562 cell line lysates (35 ug/lane). The MOB4A antibody detected the MOB4A protein (arrow).
MOB1B / MOBKL1A Antibody - Western blot of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Polyclonal Rabbit anti‑Human MOB1B / MOBKL1A Antibody (aa177‑206, IHC, IF, WB) LS‑C98948

Polyclonal Rabbit anti‑Human MOB1B / MOBKL1A Antibody (aa177‑206, IHC, IF, WB) LS‑C98948

(4.1) Rating
Submit Review (2) Publication(s) LSBio Guarantee
Antibody:
MOB1B / MOBKL1A Rabbit anti-Human Polyclonal (aa177-206) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C98948-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MOB1B / MOBKL1A Rabbit anti-Human Polyclonal (aa177-206) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MOBKL1A antibody LS-C98948 is an unconjugated rabbit polyclonal antibody to human MOBKL1A (MOB1B) (aa177-206). Validated for IF, IHC and WB. Cited in 2 publications.
(2) Publication(s)
Target
Human MOB1B / MOBKL1A
Synonyms
MOB1B | MOB kinase activator 1B | MOB4A | Mob1 homolog 1A | MOBKL1A | MATS2 | MOB1 Mps One Binder homolog B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa177-206
Specificity
This MOB4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the C-terminal region of human MOB4A.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MOB1B / MOBKL1A
NP_775739.1

LSBio Ratings

MOB1B / MOBKL1A Antibody (aa177-206) for IHC, IF/Immunofluorescence, WB/Western LS-C98948 has an LSBio Rating of
(4.1) Rating
Publications (4.1)

Publications (2)

Epigenetic regulation of the alternatively activated macrophage phenotype. Ishii M, Wen H, Corsa CA, Liu T, Coelho AL, Allen RM, Carson WF, Cavassani KA, Li X, Lukacs NW, Hogaboam CM, Dou Y, Kunkel SL. Blood. 2009 114:3244-54.
PubMed: 19567879 PMC: PMC2759649
Cancer susceptibility and embryonic lethality in Mob1a/1b double-mutant mice. Nishio M, Hamada K, Kawahara K, Sasaki M, Noguchi F, Chiba S, Mizuno K, Suzuki SO, Dong Y, Tokuda M, Morikawa T, Hikasa H, Eggenschwiler J, Yabuta N, Nojima H, Nakagawa K, Hata Y, Nishina H, Mimori K, Mori M, Sasaki T, Mak TW, Nakano T, Itami S, Suzuki A. The Journal of clinical investigation. 2012 122:4505-18.
PubMed: 23143302 PMC: PMC3533542
Submit a Publication for LS-C98948

Customer Reviews (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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