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Anti-MMP9 / Gelatinase B Antibody IHC-plus™ LS-B2486

Note: This antibody replaces LS-C8814

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular MMP9 / Gelatinase B Antibodies

Anti-MMP9 / Gelatinase B Antibody IHC-plus™ LS-B2486
Rabbit Polyclonal (IgG) to Rat MMP9 / Gelatinase B
Rat, Human, Mouse
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-MMP9 / Gelatinase B Antibody IHC-plus™ LS-B2548
Mouse Monoclonal (IgG1,k) to Human MMP9 / Gelatinase B
Human, Rat
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MMP9 / Gelatinase B Antibody (aa659-674) IHC-plus™ LS-B2807
Rabbit Polyclonal to Human MMP9 / Gelatinase B
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal (IgG) to Rat MMP9 / Gelatinase B
Rat, Human, Mouse
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated

Details

Rat MMP9 / Gelatinase B
Rabbit
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen (1:50)
  • Western blot (1:1000)
  • Immunoprecipitation (1:250)

Specificity and Use

MMP9 / Gelatinase B antibody was raised against e. coli expressed active rat 92kD type IV collagenase (catalytic domain).
Recognizes rat MMP-9 pro and active forms. Exhibits no cross-reactivity with other MMP family members.
Immunohistochemistry: LS-B2486 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2486 was determined to be 5 ug/ml. Western Blot: 1:500. Under fully reduced conditions, typically 98-95kD pro and 92-88kD active forms and breakdown products are detected. Under non-reduced conditions, additional bands may be detected due to SDS-stable TIMP and other inhibitor complexes. Positive control: HL660 cell lysate, U937 cell lysate, lung.

Packaging

PBS, 0.05% sodium azide
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (1)

1
Bevacizumab with angiostatin-armed oHSV increases antiangiogenesis and decreases bevacizumab-induced invasion in U87 glioma. Zhang W, Fulci G, Buhrman JS, Stemmer-Rachamimov AO, Chen JW, Wojtkiewicz GR, Weissleder R, Rabkin SD, Martuza RL. Molecular therapy : the journal of the American Society of Gene Therapy. 2012 Jan;20:37-45. [Full Text Article] [PubMed:21915104] [PMC:PMC3255598]

About MMP9 / Gelatinase B

P14780 NM_004994 NP_004985.2

MMP9 Antibody, 92kd gelatinase Antibody, 92 kd type iv collagenase Antibody, 92 kDa gelatinase Antibody, 92 kDa type IV collagenase Antibody, Matrix metalloproteinase-9 Antibody, MMP-9 Antibody, Type V collagenase Antibody, CLG4B Antibody, Gelatinase B Antibody, GELB Antibody, Macrophage gelatinase Antibody, MANDP2 Antibody

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens.

Immunohistochemistry

Immunohistochemistry
Anti-MMP-9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2486 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/9/2016

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