(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Paraffin (2 - 4 µg/ml)
- IHC - Frozen (2 - 4 µg/ml)
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MMP2 antibody was raised against human APMA-activated native 72kD Gelatinase A.
Recognizes proteins of 72kD and ~66kD which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72kD collagenase IV or gelatinase A). No cross-reaction with pro and active forms of other MMPs.
Immunohistochemistry (Frozen & fixed-paraffin embedded) 2-4 ug/ml g/ml 30 min at RT. Staining of formalin-fixed tissues is improved by boiling tissue sections in 10 mM citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Conditioned, serum-free medium from (dexamethasone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas. S.
10 mM PBS, pH 7.4, 15 mM Sodium Azide, 0.2% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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