Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (5 - 15 µg/ml)
Western blot (0.1 µg/ml)
Immunoprecipitation (25 µg/ml)
Specificity and Use
MMP2 antibody was raised against chinese hamster ovary cell line CHO-derived recombinant human MMP-2 Ile34-Cys660 Accession # P08253.
Detects human MMP2 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) MMP-7 and.
Western Blot, 0.1 µg/ml, Recombinant Human MMP-2 Western Blot Standard. Immunohistochemistry, 5-15 µg/ml. Immunoprecipitation, 25 µg/ml, Conditioned cell culture medium spiked with Recombinant Human MMP-2.
Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
MMP2 Antibody, 72 kd type iv collagenase Antibody, 72 kDa gelatinase Antibody, 72 kd gelatinase Antibody, 72 kDa type IV collagenase Antibody, CLG4A Antibody, CLG4 Antibody, GelA Antibody, Gelatinase A Antibody, Matrix metalloproteinase-2 Antibody, MMP-2 Antibody, MONA Antibody, Neutrophil gelatinase Antibody, TBE-1 Antibody, Tbe-1 metalloprotease Antibody, Collagenase type IV-A Antibody, Matrix metalloproteinase-II Antibody, MMP-II Antibody
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways.
MMP-2 in Human Ovary. M M P-2 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Human MMP-2 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.
MMP-2 in Human Ovarian Cancer Tissue. M M P-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP-2 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).
Requested From: United States
Date Requested: 1/21/2017