Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse (tested or 100% immunogen sequence identity)
Protein A purified
IHC - Frozen (1:20 - 1:50), Western blot (1:250 - 1:500)
Specificity and Use
MMP16 antibody was raised against synthetic peptide derived from hinge region of human MMP16.
Recognizes the human membrane bound matrix metalloproteinase designated MMP16 (MT3-MMP). The antibody binds to an epitope in the hinge region of the protein and shows no cross-reactivity with other members of the MMP family. Species cross-reactivity: Human, mouse.
Immunohistochemistry: (frozen)- 1:20-1:50. Western Blot: 1:250-1:500.
PBS, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
MMP16 is an endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.