MMP14 antibody LS-C8914 is an unconjugated mouse monoclonal antibody to MMP14 from human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
Human, Monkey, Mouse, Rat, Bovine, Goat, Hamster, Sheep
(tested or 100% immunogen sequence identity)
Bat, Dog, Horse, Rabbit, Chicken (at least 90% immunogen sequence identity)
- IHC - Paraffin
- IHC - Frozen
- Western blot (10 µg/ml)
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MMP14 antibody was raised against peptide (aa 319-333, CDGNFDTVAMLRGEM) in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP also referred to as MMP-14. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%); Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%); Zebrafish, Xenopus, Medaka (87%); Lizard (80%).
Recognizes human MT1-MMP. Very slight cross-reactivity to hMMP-3. Species cross-reactivity: Mouse, rat, rabbit porcine and equine. For unknown reasons this antibody does not react on westerns to MMP-14 [MT1-MMP], Human, Prodomain, Catalytic Domain.
Suitable for use in ELISA, Western Blot and Immunohistochemistry, and Immunoprecipitation. Western Blot: 10 ug/ml, membrane preparations; MD-MB-231 cells pretreated with 1-5 ug/ml ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections; traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required.
PBS, pH 7.0, 2% BSA (protease free).
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.