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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MMP14 Antibody (aa319‑333) LS‑C8914
MMP14 antibody LS-C8914 is an unconjugated mouse monoclonal antibody to MMP14 from human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C8914-100
100 µg (2 mg/ml)
$665

Popular MMP14 Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-MMP14 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3338 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MMP-14 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-MMP14 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8126 dilution 1:50.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Confocal immunofluorescence of MMP14 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Species: Human
Applications: Immunofluorescence, Western blot

Product Description

MMP14 antibody LS-C8914 is an unconjugated mouse monoclonal antibody to MMP14 from human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
About MMP14
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. P50281 NM_004995 NP_004986.1

MMP14 Antibody, Membrane type-1 MMP Antibody, MT1-MMP Antibody, MT1MMP Antibody, MT-MMP Antibody, MT-MMP 1 Antibody, Matrix metalloproteinase-14 Antibody, MMP-14 Antibody, MMP-X1 Antibody, MTMMP1 Antibody


Specifications

Target
Human MMP14
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Goat, Hamster, Sheep (tested or 100% immunogen sequence identity)
Predicted
Bat, Dog, Horse, Rabbit, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG3,k Monoclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Western blot (10 µg/ml)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MMP14 antibody was raised against peptide (aa 319-333, CDGNFDTVAMLRGEM) in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP also referred to as MMP-14. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%); Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%); Zebrafish, Xenopus, Medaka (87%); Lizard (80%).
Epitope
aa319-333
Specificity
Recognizes human MT1-MMP. Very slight cross-reactivity to hMMP-3. Species cross-reactivity: Mouse, rat, rabbit porcine and equine. For unknown reasons this antibody does not react on westerns to MMP-14 [MT1-MMP], Human, Prodomain, Catalytic Domain.
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry, and Immunoprecipitation. Western Blot: 10 ug/ml, membrane preparations; MD-MB-231 cells pretreated with 1-5 ug/ml ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections; traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required.
Presentation
PBS, pH 7.0, 2% BSA (protease free).
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/14/2018

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