(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin (10 - 20 µg/ml)
- Western blot (2.5 - 5 µg/ml)
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MMP13 antibody was raised against human recombinant MMP-13 protein.
Recognizes human MMP-13. Species cross-reactivity: rat.
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 2.5-5 ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 10-20 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Conditioned, serum-free medium from (TPA-treated) rat CCL-4 cells. Placenta.
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 40% Glycerol, 0.2% BSA
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.