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Anti-MMP13 Antibody LS-C8867

Ordering

Wt. Vol. Conc. Price
- 250 µl 0.5 mg/ml $375
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Rabbit Polyclonal (IgG) to Human MMP13
Human, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human MMP13
Rabbit
Human, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified

Applications

IHC - Paraffin (10 - 20 µg/ml), Western blot (2.5 - 5 µg/ml)

Specificity and Use

MMP13 antibody was raised against human recombinant MMP-13 protein.
Recognizes human MMP-13. Species cross-reactivity: rat.
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 2.5-5 ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 10-20 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Conditioned, serum-free medium from (TPA-treated) rat CCL-4 cells. Placenta.

Packaging

10 mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, 40% glycerol.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About MMP13

P45452 NM_002427 NP_002418.1

MMP13 Antibody, Collagenase 3 Antibody, Matrix metalloproteinase-13 Antibody, Interstitial collagenase Antibody, MANDP1 Antibody, MMP-13 Antibody, CLG3 Antibody

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF.

Requested From: United States
Date Requested: 9/25/2016

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