(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin (10 - 20 µg/ml)
- Western blot (2.5 - 5 µg/ml)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
MMP13 antibody was raised against human recombinant MMP-13 protein.
Recognizes human MMP-13. Species cross-reactivity: rat.
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 2.5-5 ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 10-20 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Conditioned, serum-free medium from (TPA-treated) rat CCL-4 cells. Placenta.
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 40% Glycerol, 0.2% BSA
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.