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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MME / CD10 Antibody (aa506‑534) LS‑C164829
CD10 antibody LS-C164829 is an unconjugated rabbit polyclonal antibody to human CD10 (MME). Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C164829-400
400 µl
Unavailable

Popular MME / CD10 Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
Antibody
Species: Rat, Human, Mouse
Applications: IHC, Western blot
Antibody
Species: Human
Applications: Western blot, Flow Cytometry
Antibody
Species: Human
Applications: Immunofluorescence, Flow Cytometry
Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin

Product Description

CD10 antibody LS-C164829 is an unconjugated rabbit polyclonal antibody to human CD10 (MME). Validated for Flow, IHC and WB.
About MME / CD10
MME / CD10 is a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. P08473 NM_000902 NP_000893.2

MME Antibody, Atriopeptidase Antibody, CALLA Antibody, CD10 Antibody, EPN Antibody, Membrane metallo-endopeptidase Antibody, NEP Antibody, Neprilysin Antibody, Neutral endopeptidase Antibody, Neutral endopeptidase 24.11 Antibody, CD10 antigen Antibody, Enkephalinase Antibody, Neprylisin Antibody, SFE Antibody, Skin fibroblast elastase Antibody


Specifications

Target
Human MME / CD10
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8867 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C164829. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa506-534
Specificity
This Neprilysin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human Neprilysin.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Customer Reviews (0)


Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human kidney carcinoma with Neprilysin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human kidney carcinoma with Neprilysin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of Neprilysin Antibody in A2058,A375,Ramos cell line lysates (35 ug/lane). EPN (arrow) was detected using the purified antibody.
Western blot of Neprilysin Antibody in A2058,A375,Ramos cell line lysates (35 ug/lane). EPN (arrow) was detected using the purified antibody.

Flow Cytometry

Neprilysin Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Neprilysin Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human kidney carcinoma with Neprilysin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human kidney carcinoma with Neprilysin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of Neprilysin Antibody in A2058,A375,Ramos cell line lysates (35 ug/lane). EPN (arrow) was detected using the purified antibody.
Western blot of Neprilysin Antibody in A2058,A375,Ramos cell line lysates (35 ug/lane). EPN (arrow) was detected using the purified antibody.

Flow Cytometry

Neprilysin Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Neprilysin Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human kidney carcinoma with Neprilysin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human kidney carcinoma with Neprilysin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of Neprilysin Antibody in A2058,A375,Ramos cell line lysates (35 ug/lane). EPN (arrow) was detected using the purified antibody.
Western blot of Neprilysin Antibody in A2058,A375,Ramos cell line lysates (35 ug/lane). EPN (arrow) was detected using the purified antibody.

Flow Cytometry

Neprilysin Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Neprilysin Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human kidney carcinoma with Neprilysin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human kidney carcinoma with Neprilysin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of Neprilysin Antibody in A2058,A375,Ramos cell line lysates (35 ug/lane). EPN (arrow) was detected using the purified antibody.
Western blot of Neprilysin Antibody in A2058,A375,Ramos cell line lysates (35 ug/lane). EPN (arrow) was detected using the purified antibody.

Flow Cytometry

Neprilysin Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Neprilysin Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/24/2018

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