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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MLX / TCFL4 Antibody (aa201‑250) LS‑C80294
TCFL4 antibody LS-C80294 is an unconjugated rabbit polyclonal antibody to TCFL4 (MLX) from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C80294-50
50 µg
Unavailable

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Antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

TCFL4 antibody LS-C80294 is an unconjugated rabbit polyclonal antibody to TCFL4 (MLX) from human, mouse, rat and other species. Validated for IHC and WB.
About MLX / TCFL4
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation. Q9UH92 NM_170607 NP_733752.1

MLX Antibody, BHLHd13 Antibody, BigMax protein Antibody, MXD7 Antibody, MAD7 Antibody, Max-like bHLHZip protein Antibody, TCFL4 Antibody, MLX, MAX dimerization protein Antibody, Protein BigMax Antibody, Max-like protein X Antibody, Transcription factor-like 4 Antibody


Specifications

Target
Human MLX / TCFL4
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Hamster, Horse, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MLX / TCFL4 antibody was raised against synthetic peptide located between aa201-250 of human MLX (Q9UH92, NP_733752). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Turkey, Chicken (100%); Lizard (92%); Platypus, Stickleback (85%); Zebrafish (84%).
Blocking Peptide
LS-E14526 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80294. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa201-250
Specificity
Human MLX
Usage
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

MLX antibody LS-C80294 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MLX antibody LS-C80294 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

MLX antibody LS-C80294 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MLX antibody LS-C80294 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

MLX antibody LS-C80294 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MLX antibody LS-C80294 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

MLX antibody LS-C80294 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MLX antibody LS-C80294 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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