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Anti-MLH1 Antibody (clone 4C9C7) IHC-plus™ LS-B3475

Note: This antibody replaces LS-C102880
Catalog Size Price
LS-B3475-50 50 µl Unavailable

Related Products

46 MLH1 Antibodies

Most Popular MLH1 Antibodies

Anti-MLH1 Antibody (clone G168-15) LS-C95607
Mouse Monoclonal [clone G168-15] (IgG1)
Human, Rat, Hamster
IHC, IHC - Paraffin
Unconjugated
Anti-MLH1 Antibody (clone 4C9C7) IHC-plus™ LS-B3475
Mouse Monoclonal [clone 4C9C7] (IgG1) to Human MLH1
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-MLH1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3475 dilution 1:200.
Anti-MLH1 Antibody LS-C109333
Rabbit Polyclonal to Human MLH1
Human
ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence of paraformaldehyde-fixed HeLa, using MLH1 antibody at 1:200 dilution.
Anti-MLH1 Antibody (clone M1) LS-C133324
Mouse Monoclonal [clone M1] (IgG) to Human MLH1
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence of monoclonal antibody to MLH1 on HeLa cell. [antibody concentration 10 ug/ml]
Anti-MLH1 Antibody (Internal) LS-C210492
Rabbit Polyclonal (IgG) to Human MLH1
Human
IHC, IHC - Paraffin
Unconjugated
Immunohistochemistry of Human Colon Carcinoma stained with anti-MLH1 antibody

100% Guaranteed
Mouse Monoclonal [clone 4C9C7] (IgG1) to Human MLH1
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human MLH1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [4C9C7]
Unconjugated
Ascites
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)

Specificity and Use

MLH1 antibody was raised against purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.\
Human, Monkey
Immunohistochemistry: LS-B3475 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3475 was determined to be 1:200.

Packaging

Ascitic fluid containing 0.03% sodium azide
Short term: 4°C; Long term: -20°C.
For research use only.

About MLH1

P40692 NM_000249 NP_000240.1

MLH1 Antibody, COCA2 Antibody, FCC2 Antibody, HMLH1 Antibody, HNPCC Antibody, HNPCC2 Antibody, MutL protein homolog 1 Antibody

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.

Immunohistochemistry

Anti-MLH1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3475 dilution 1:200.
Anti-MLH1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3475 dilution 1:200.

Immunofluorescence

Confocal immunofluorescence of HeLa cells using MLH1 mouse monoclonal antibody (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Confocal immunofluorescence of HeLa cells using MLH1 mouse monoclonal antibody (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using MLH1 mouse monoclonal antibody against HeLa (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.
Western blot using MLH1 mouse monoclonal antibody against HeLa (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.

Requested From: 
Date Requested: 5/27/2018

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