Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
MLH1, COCA2, FCC2, HMLH1, HNPCC, HNPCC2, MutL protein homolog 1
(tested or 100% immunogen sequence identity)
IHC - Frozen
Western blot (1:500 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
MLH1 antibody was raised against synthetic peptide corresponding to a region of human MLH1 encoded within exon 20.
Specific for human MLH1 (Homologue 1 of E. coli MutL Protein).
Suitable for use in Western Blot. Immunoprecipitation and Immunohistochemistry (Frozen). Western Blot: 1:500-1:5000. Immunoprecipitation:1-4 ug/mg protein.
Tris-citrate/phosphate, 0.02% sodium azide.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.