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Anti-MLC2 / MYL9 Antibody (Ser18) IHC-plus™ LS-B1481

Note: This antibody replaces LS-C34375

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MLC2 / MYL9 Antibodies

Anti-MLC2 / MYL9 Antibody (aa12-27) IHC-plus™ LS-B38
Rabbit Polyclonal to Human MLC2 / MYL9
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MLC2 / MYL9 Antibody (C-Terminus) IHC-plus™ LS-B5814
Rabbit Polyclonal (IgG) to Human MLC2 / MYL9
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MLC2 / MYL9 Antibody LS-C332334
Rabbit Polyclonal (IgG) to Human MLC2 / MYL9
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse MLC2 / MYL9
Mouse, Human, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Mouse MLC2 / MYL9
Rabbit
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot

Specificity and Use

MLC2 / MYL9 antibody was raised against synthetic peptide from mouse MYL9.
Ser18
Amino acids surrounding Ser 18 (ATSNV) of mouse MYL2
Immunohistochemistry: LS-B1481 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1481 was determined to be 10 ug/ml.

Packaging

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About MLC2 / MYL9

P24844 NM_006097 NP_006088.2

MYL9 Antibody, 20 kDa myosin light chain Antibody, LC20 Antibody, MLC-2C Antibody, MLC2 Antibody, Myosin RLC Antibody, MRLC1 Antibody, MYRL2 Antibody

Myosin, the major component of muscle filaments, is a long asymmetric molecule containing a globular head and a long tail. It is composed of two heavy chains and four light chains. Phosphorylation of myosin light chain in response to increased calcium levels leads to contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.

Immunohistochemistry

Immunohistochemistry
Anti-MYL9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1481 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using Myosin Light Chain 2(Ab-18) antibody.

Western blot

Western blot
Western blot of extracts from K562 cells using Myosin Light Chain 2(Ab-18) antibody and the same antibody preincubated with blocking peptide.
 

Requested From: United States
Date Requested: 12/4/2016

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