Human MKI67 / Ki67

Rabbit

Human (tested or 100% immunogen sequence identity)

Rat

IgG Polyclonal

Unconjugated

Protein A purified

Unmodified

Synthetic peptide corresponding to an internal region of human Ki-67 (within residues 1550-1700). Cellular Localization: Nuclear

Recognizes human Ki-67. Reacts with a nuclear antigen present in proliferating human cells. Can be used to evaluate the Ki-67 labeling index in various tumors.

• IHC
• IHC - Paraffin
• IHC - Frozen
• ICC (1:50)
• Immunofluorescence (1:20)

Suitable for use in Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Immunohistochemistry (Frozen/Paraffin): Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. We suggest an incubation period of 30 minutes at RT and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear). Immunocytochemistry: 1:50. Immunofluorescence: 1:20. Positive control: Tonsil.

PBS, 0.05% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P46013 NCBI: NM_002417 NP_002408.3