Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Protein A purified
IHC - Paraffin
IHC - Frozen
Specificity and Use
MKI67 / Ki67 antibody was raised against synthetic peptide corresponding to an internal region of human Ki-67 (within residues 1550-1700). Cellular Localization: Nuclear
Recognizes human Ki-67. Reacts with a nuclear antigen present in proliferating human cells. Can be used to evaluate the Ki-67 labeling index in various tumors.
Suitable for use in Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Immunohistochemistry (Frozen/Paraffin): Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. We suggest an incubation period of 30 minutes at RT and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear). Immunocytochemistry: 1:50. Immunofluorescence: 1:20. Positive control: Tonsil.
PBS, 0.05% sodium azide, 30% glycerol
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.