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Anti-MITF Antibody LS-C150000

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular MITF Antibodies

Anti-MITF Antibody (aa1-28) LS-C164206
Rabbit Polyclonal to Human MITF
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunofluorescence Image
Anti-MITF Antibody (aa1-28, PE) LS-C263189
Rabbit Polyclonal (IgG) to Human MITF
Human
ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human MITF
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human MITF
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

MITF antibody was raised against e. coli-derived recombinant human MITF Val1 19-Lys289 Accession # O75030.
Detects human MITF in direct ELISAs and Western blots.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About MITF

O75030 NM_000248 NP_000239.1

MITF Antibody, BHLHe32 Antibody, CMM8 Antibody, MI Antibody, Waardenburg syndrome, type 2A Antibody, WS2 Antibody, WS2A Antibody

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1).

Immunohistochemistry

Immunohistochemistry
MITF in Human Skin. MITF was detected in paraffin-embedded sections of human skin using Goat Anti-Human MITF Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).

Western blot

Western blot
Detection of Human MITF by Western Blot. Western blot shows lysates of Bowes human melanoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human MITF Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for MITF at approximately 60 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/7/2016

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