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Anti-Mineralocorticoid Receptor Antibody (clone H10E4C9F) IHC-plus™ LS-B3162

Note: This antibody replaces LS-C25630

Ordering

Wt. Vol. Conc. Price
- 50 µl 1.4 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Mineralocorticoid Receptor Antibodies

Anti-Mineralocorticoid Receptor Antibody (clone H10E4C9F) IHC-plus™ LS-B3162
Mouse Monoclonal [clone H10E4C9F] (IgG1) to Human Mineralocorticoid Receptor
Human, Rat, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-Mineralocorticoid Receptor Antibody (N-Terminus) LS-C107485
Rabbit Polyclonal (IgG) to Human Mineralocorticoid Receptor
Human, Mouse, Rat, Bovine, Horse, Pig
Western blot
Unconjugated
Western blot Image
Anti-Mineralocorticoid Receptor Antibody (C-Terminus) LS-C112346
Rabbit Polyclonal to Human Mineralocorticoid Receptor
Human, Monkey, Mouse, Rat, Bat, Horse, Chicken
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone H10E4C9F] (IgG1) to Human Mineralocorticoid Receptor
Human, Rat, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated

Details

Human Mineralocorticoid Receptor
Mouse
Human, Rat, Rabbit, Chicken (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [H10E4C9F]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Western blot (1:250)

Specificity and Use

Mineralocorticoid Receptor antibody was raised against aldosterone.
Recognizes human MR (mineralocorticoid receptor). Detects a band of ~116kD by Western Blot. Species cross-reactivity: Rat, rabbit and chicken.
Immunohistochemistry: LS-B3162 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3162 was determined to be 1:50. Function: Blocks the binding of aldosterone to the MR.

Packaging

PBS, pH 7.2, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Mineralocorticoid Receptor

P08235 NM_000901 NP_000892.2

NR3C2 Antibody, MCR Antibody, Mineralocorticoid receptor Antibody, NR3C2VIT Antibody, Aldosterone receptor Antibody, Mineralocorticoid receptor 1 Antibody, MLR Antibody, MR Antibody

The mineralocorticoid receptor (MR), a NR3 Steroid Receptor, has been shown to affect hypothalamic-pituitary-adrenocortical axis activity in the brain, sodium and potassium transport, and stress-related psychiatric diseases such as major depression. MR binds aldosterone and glucocorticoid with equal affinity, and the binding specificity is regulated by the enzyme 11beta-hydroxysteroid-dehydrogenase type II.

Immunohistochemistry

Immunohistochemistry
Anti-NR3C2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3162 dilution 1:50.

Immunohistochemistry

Immunohistochemistry
Anti-NR3C2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3162 dilution 1:50.

Requested From: United States
Date Requested: 12/10/2016

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