Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The mineralocorticoid receptor (MR), a NR3 Steroid Receptor, has been shown to affect hypothalamic-pituitary-adrenocortical axis activity in the brain, sodium and potassium transport, and stress-related psychiatric diseases such as major depression. MR binds aldosterone and glucocorticoid with equal affinity, and the binding specificity is regulated by the enzyme 11beta-hydroxysteroid-dehydrogenase type II.
Detection of Human Mineralocorticoid R/NR3C2 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 ug/ml of Human Mineralocorticoid R/NR3C2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Mineralocorticoid R/NR3C2 at approximately 110 kD (as indicated). This experiment was conducted under reducing conditions.