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Anti-MID1 Antibody (aa57-106) LS-C187219

Catalog	Size	Price
LS-C187219-50	50 µg (1 mg/ml)	Unavailable

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29 MID1 Antibodies

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Anti-MID1 Antibody (aa71-120) LS-C118672

Antibody:	Rabbit Polyclonal (IgG) to Human MID1
Reactivity:	Human, Mouse, Rat
Application:	IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Format:	Unconjugated

IHC of paraffin-embedded human brain tissue, using TRI18 Antibody. The picture on the right is treated with the synthesized peptide.

Anti-MID1 Antibody (aa40-120) LS-C384537

Antibody:	Rabbit Polyclonal (IgG) to Human MID1
Reactivity:	Human, Mouse, Rat
Application:	IHC, Immunofluorescence, Western blot, ELISA
Format:	Unconjugated

Anti-MID1 Antibody LS-C412832

Antibody:	Rabbit Polyclonal to Human MID1
Reactivity:	Human, Rat
Application:	IHC, Immunofluorescence, Western blot
Format:	Unconjugated

Anti-MID1 Antibody (N-Terminus) LS-C413053

Antibody:	Rabbit Polyclonal to Human MID1
Reactivity:	Human, Monkey, Mouse, Rat, Dog
Application:	IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Format:	Unconjugated

Immunohistochemical analysis of MID1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Anti-MID1 Antibody LS-C416014

Antibody:	Rabbit Polyclonal to Human MID1
Reactivity:	Human, Mouse, Rat
Application:	IHC, ICC, Immunofluorescence, Western blot
Format:	Unconjugated

Western blot of A549 and HUVEC cell lysate using TRI18 Antibody

Antibody:	Rabbit Polyclonal to Human MID1
Reactivity:	Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Chicken, Xenopus, Zebrafish
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Western blot of Human Transfected 293T. MID1 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Details

Target:	Human MID1
Host:	Rabbit
Reactivity:	Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Predicted:	Rabbit (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

MID1 antibody was raised against synthetic peptide located between aa57-106 of human MID1 (O15344-2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (100%); Rabbit (92%); Hamster (85%).

Blocking Peptide:

LS-E21234 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C187219. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa57-106

Specificity:

Human OSX / MID1

Packaging

Presentation:	Lyophilized from PBS
Reconstitution:	50 µl Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About MID1

Uniprot: O15344 NCBI: NM_000381 NP_000372.1

Synonyms: MID1 Antibody, GBBB1 Antibody, Midline 1 RING finger protein Antibody, MIDIN Antibody, RING finger protein 59 Antibody, RNF59 Antibody, Midline 1 (Opitz/BBB syndrome) Antibody, TRIM18 Antibody, OGS1 Antibody, ZNFXY Antibody, BBBG1 Antibody, XPRF Antibody, FXY Antibody, Midline-1 Antibody, OS Antibody, OSX Antibody

Description: The MID1 gene (also called OGS1 and FXY) is ubiquitously expressed in both fetal and adult tissues and displays a transcript of approximately 7 kb that encodes a 667-amino acid protein. The 10-exon human MID1 gene is a member of the RING finger family, characterized by the presence of an N-terminal zinc binding domain.

(More About MID1)

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Western blot

Western blot of Human Transfected 293T. MID1 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 4/20/2018

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