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Anti-MICA Antibody (aa68-97) IHC-plus™ LS-B9176

Note: This antibody replaces LS-C164186
Catalog Size Price
LS-B9176-200 200 µl Unavailable

Related Products

41 MICA Antibodies

Most Popular MICA Antibodies

Anti-MICA Antibody (aa201-250) LS-C40461
Rabbit Polyclonal (IgG) to Human MICA
Human
Western blot
Unconjugated
Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-MICA Antibody (C-Terminus) IHC-plus™ LS-B1377
Rabbit Polyclonal (IgG) to Human MICA
Human, Mouse
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-MICA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1377 concentration 5 ug/ml.
Anti-MICA Antibody (Biotin) LS-C124112
Mouse Monoclonal (IgG2a) to Human MICA
Human
IHC, IHC - Frozen, Flow Cytometry
Biotin Conjugated
Anti-MICA Antibody (aa23-308) LS-C149998
Goat Polyclonal (IgG) to Human MICA
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
MICA in Human Breast Cancer Tissue. MICA was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human MICA Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4 °C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).
Anti-MICA Antibody (aa68-97, Biotin) LS-C279575
Rabbit Polyclonal (IgG) to Human MICA
Human
IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Biotin Conjugated

100% Guaranteed
Rabbit Polyclonal to Human MICA
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human MICA
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E9684 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9176. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa68-97
This MICA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human MICA.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About MICA

Q29983 NM_000247 NP_000238.1

MICA Antibody, HLA class I antigen Antibody, MIC-A Antibody, PERB11.1 Antibody

MICA is the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

Immunohistochemistry

Anti-MICA antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MICA antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Confocal immunofluorescence of MICA Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of MICA Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of MICA Antibody in MDA-MB231 cell line lysates (35 ug/lane). MICA (arrow) was detected using the purified antibody.
Western blot of MICA Antibody in MDA-MB231 cell line lysates (35 ug/lane). MICA (arrow) was detected using the purified antibody.

Western blot

MICA Antibody western blot of MDA-MB-231 cell line lysates (35 ug/lane). The MICA antibody detected the MICA protein (arrow).
MICA Antibody western blot of MDA-MB-231 cell line lysates (35 ug/lane). The MICA antibody detected the MICA protein (arrow).

Flow Cytometry

Flow cytometric of SK-Br-3 cells using MICA Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of SK-Br-3 cells using MICA Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 4/20/2018

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