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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MIB1 Antibody (clone 3E10) LS‑C139207
MIB1 antibody LS-C139207 is an unconjugated mouse monoclonal antibody to human MIB1. Validated for ELISA.
Catalog
Size
Price
LS-C139207-100
100 µg
Unavailable

Popular MIB1 Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Western blot of HMGB1 antibody at 2ug/ml + 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 111 kDa. Observed band size: 111 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot, ELISA
Western blot of HMGB1 antibody at 2ug/ml + 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 111 kDa. Observed band size: 111 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
Immunocytochemistry of MIB1 in A431 cells with MIB1 antibody at 5 µg/mL.
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot, ELISA

Product Description

MIB1 antibody LS-C139207 is an unconjugated mouse monoclonal antibody to human MIB1. Validated for ELISA.
About MIB1
E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis By similarity. Q86YT6 AB037744 BAA92561.1

MIB1 Antibody, DIP-1 Antibody, DIP1 Antibody, Mindbomb homolog 1 Antibody, KIAA1323 Antibody, Mind bomb homolog 1 Antibody, Ubiquitin ligase protein MIB1 Antibody, Ubiquitin ligase mind bomb Antibody, ZZZ6 Antibody, DAPK-interacting protein 1 Antibody, Mib Antibody, ZZANK2 Antibody


Specifications

Target
Human MIB1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [3E10]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • ELISA
Immunogen
MIB1 antibody was raised against mIB1 (NP_065825, 909 a.a. ~ 1007 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human MIB1
Usage
Sandwich ELISA: Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged MIB1 is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MIB1 is 3 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged MIB1 is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MIB1 is 3 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged MIB1 is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MIB1 is 3 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged MIB1 is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MIB1 is 3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/23/2018

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