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Anti-MIA40 / CHCHD4 Antibody (N-Terminus) LS-C102407

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

Related Products

22 MIA40 / CHCHD4 Antibodies

Most Popular MIA40 / CHCHD4 Antibodies

Anti-MIA40 / CHCHD4 Antibody (N-Terminus) LS-C153493
Rabbit Polyclonal to Human MIA40 / CHCHD4
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Anti-MIA40 / CHCHD4 Antibody (aa101-129) LS-C164184
Rabbit Polyclonal to Human MIA40 / CHCHD4
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MIA40 / CHCHD4 Antibody (aa1-142, HRP) LS-C371869
Rabbit Polyclonal (IgG) to Human MIA40 / CHCHD4
Human
IHC, IHC - Paraffin, ELISA
HRP Conjugated
Immunohistochemistry Image
Anti-MIA40 / CHCHD4 Antibody (aa1-142, Biotin) LS-C371870
Rabbit Polyclonal (IgG) to Human MIA40 / CHCHD4
Human
IHC, IHC - Paraffin, ELISA
Biotin Conjugated
Immunohistochemistry Image
Anti-MIA40 / CHCHD4 Antibody (aa1-142, FITC) LS-C371871
Rabbit Polyclonal (IgG) to Human MIA40 / CHCHD4
Human
IHC, IHC - Paraffin, ELISA
FITC Conjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human MIA40 / CHCHD4
Human, Mouse, Rat, Guinea pig, Chicken, Xenopus
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human MIA40 / CHCHD4
Rabbit
Human, Mouse, Rat, Guinea pig, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Monkey, Bovine, Dog, Horse, Pig, Rabbit, Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

MIA40 / CHCHD4 antibody was raised against synthetic peptide from N-Terminus of human CHCHD4 (Q8N4Q1, NP_001091972). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Mouse, Rat, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus (100%); Monkey, Marmoset, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Zebra finch, Seabass (92%); Zebrafish (90%); Salmon, Sablefish, Stickleback (85%).
LS-E18620 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102407. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
N-Terminus
Human CHCHD4 / MIA40
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MIA40 / CHCHD4

Q8N4Q1 Q8N4Q1

CHCHD4 Antibody, CHDH4 Antibody, TIMM40 Antibody, MIA40 Antibody

Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria.

Western blot

Western blot
CHCHD4 / MIA40 antibody LS-C102407 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/23/2017

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