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MIA40 / CHCHD4

coiled-coil-helix-coiled-coil-helix domain containing 4

Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system.

Gene Name: coiled-coil-helix-coiled-coil-helix domain containing 4
Synonyms: CHCHD4, CHDH4, TIMM40, MIA40
Target Sequences: Q8N4Q1 Q8N4Q1

Publications (1)

1
Rapid enrichment of mitochondria from mammalian cell cultures using digitonin. Bhavna Dixit, Shon Vanhoozer, Nana Abena Anti, Matthew S O'Connor, Amutha Boominathan. MethodsX. 2020 Dec;8:101197. [Full Text Article] [PubMed:34434723] [PMC:PMC8374178] Related Antibodies: LS-C802857.

☰ Filters
Antibodies (21)
Proteins (5)
Over-Expression Lysate (2)
Primary (21)
Recombinant (3)
MIA40 / CHCHD4 (26)
Human (21)
Mouse (6)
Rat (5)
Chicken (3)
Chimpanzee (3)
Gibbon (3)
Guinea pig (3)
Xenopus (3)
IHC (12)
IHC-P (6)
WB (14)
ELISA (10)
IF (1)
IP (2)
Peptide-ELISA (1)
rabbit (21)
IgG (20)
polyclonal pc (21)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Preservative Free (1)
Unconjugated (10)
aa101-129 (7)
N-Terminus (4)
aa1-142 (4)
aa92-142 (2)
No (25)
Yes (1)
GST (1)
His-SUMO (1)
Myc-DDK (Flag) (3)
Human (4)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (1)
MIA40 / CHCHD4 Antibody - 1:100 staining human lymph carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
MIA40 / CHCHD4 Antibody - Immunohistochemistry of paraffin-embedded human liver tissue using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa1-142) Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µg/$294; 100 µg/$360
MIA40 / CHCHD4 Antibody - Immunohistochemistry of paraffin-embedded human liver tissue using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa1-142) (HRP) Antibody
Human
ELISA, IHC, IHC-P
HRP Conjugated
50 µg/$294; 100 µg/$360
MIA40 / CHCHD4 Antibody - Immunohistochemistry of paraffin-embedded human liver tissue using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa1-142) (Biotin) Antibody
Human
ELISA, IHC, IHC-P
Biotin Conjugated
50 µg/$294; 100 µg/$360
MIA40 / CHCHD4 Antibody - Immunohistochemistry of paraffin-embedded human liver tissue using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa1-142) (FITC) Antibody
Human
ELISA, IHC, IHC-P
FITC Conjugated
50 µg/$294; 100 µg/$360
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa101-129) Antibody
Human
ELISA, IHC, WB
Unconjugated
200 µl/$734
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa101-129) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa101-129) (FITC) Antibody
Human
ELISA, IHC, WB
FITC Conjugated
200 µl/$952
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa101-129) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa101-129) (PE) Antibody
Human
ELISA, IHC, WB
PE Conjugated
200 µl/$952
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa101-129) (Azide-free) (HRP) Antibody
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
MIA40 / CHCHD4 Antibody - MIA40 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa101-129) Antibody
Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
MIA40 / CHCHD4 Antibody - Immunochemical staining CHCHD4 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
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MIA40 / CHCHD4 Rabbit anti-Mouse Polyclonal (Preservative Free) Antibody
Mouse, Rat, Human
IHC-P
Unconjugated, Preservative Free
100 µl/$412
MIA40 / CHCHD4 Antibody - Detection of human CHCHD4 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHCHD4 antibody used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa92-142) Antibody
Human
IP, WB
Unconjugated
10 µl/$295
MIA40 / CHCHD4 Antibody - CHCHD4 / MIA40 antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$424
MIA40 / CHCHD4 Antibody - CHCHD4 / MIA40 antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (N-Terminus) (HRP) Antibody
Xenopus, Chicken, Mouse, Guinea pig, Rat, Chimpanzee, Gibbon, Human
WB
HRP Conjugated
100 µl/$469
MIA40 / CHCHD4 Antibody - CHCHD4 / MIA40 antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (N-Terminus) (Biotin) Antibody
Xenopus, Chicken, Mouse, Guinea pig, Rat, Chimpanzee, Gibbon, Human
WB
Biotin Conjugated
100 µl/$469
MIA40 / CHCHD4 Antibody - CHCHD4 / MIA40 antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (N-Terminus) (FITC) Antibody
Xenopus, Chicken, Mouse, Guinea pig, Rat, Chimpanzee, Gibbon, Human
WB
FITC Conjugated
100 µl/$469
MIA40 / CHCHD4 Antibody - Western blot analysis of extracts of various cell lines, using CHCHD4 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
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MIA40 / CHCHD4 Rabbit anti-Human Polyclonal Antibody
Human
Unconjugated
100 µg/$331
Select
MIA40 / CHCHD4 Rabbit anti-Human Polyclonal (aa92-142) Antibody
Human
IP
Unconjugated
10 µl/$295
MIA40 / CHCHD4 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
15.8 kDa
20 µg/$150
MIA40 / CHCHD4 Protein
Select
E. coli
His-SUMO
32 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
MIA40 / CHCHD4 Protein - 12.5% SDS-PAGE of human CHCHD4 stained with Coomassie Blue
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Wheat Germ Extract
GST
42.4 kDa
10 µg/$479; 25 µg/$670
MIA40 / CHCHD4 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
15.8 kDa
100 µg/$494
Viewing 1-25 of 26 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).