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Anti-MIA2 Antibody LS-C104283

Ordering

Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MIA2 Antibodies

Anti-MIA2 Antibody (aa509-558) LS-C135871
Rabbit Polyclonal to Human MIA2
Human, Monkey
Western blot
Unconjugated
Western blot Image
Anti-MIA2 Antibody (aa509-558, Biotin) LS-C467893
Rabbit Polyclonal to Human MIA2
Human, Gibbon, Monkey
Western blot
Biotin Conjugated
Western blot Image
Anti-MIA2 Antibody (aa509-558, HRP) LS-C467895
Rabbit Polyclonal to Human MIA2
Human, Gibbon, Monkey
Western blot
HRP Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human MIA2
Human
IHC - Paraffin, Western blot, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Human MIA2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)

Specificity and Use

MIA2 antibody was raised against e.coli derived recombinant Human MIA-2.
Human MIA-2
Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary. ELISA: To detect hMIA-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIA-2 (LS-C104686) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIA-2. Western Blot: To detect hMIA-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About MIA2

Q96PC5 NM_054024 NP_473365.3

MIA2 Antibody, Melanoma inhibitory activity 2 Antibody

MIA2 / Melanoma inhibitory activity 2 may play a role in the pathophysiology of liver disease and may serve as a marker of liver damage. It is expressed in highly liver and weakly in testis. Expression is higher in patients with severe fibrosis or inflammation and chronic hepatitis.

Immunohistochemistry

Immunohistochemistry
IHC of MIA2 antibody LS-C104283. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
IHC of MIA2 antibody LS-C104283. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of MIA2 antibody LS-C104283. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of MIA2 antibody LS-C104283. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of MIA2 antibody LS-C104283. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/3/2016

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