Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone OX-3] (IgG1) to Rat MHC Class II RT1.Bu
Rat MHC Class II RT1.Bu
Rat, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [OX-3]
Flow Cytometry (1:1)
Specificity and Use
Rat thymocyte membrane glycoproteins.
Recognizes a polymorphic determinant of rat Ia antigen (RT1. Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. Is useful for distinguishing Ia positive cells from different rat strains, e. g. for recognizing cells of donor origin in bone marrow reconstituted radiation chimaeras. MRC OX-3 Reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Recognizes Ia antigens on B-cells, dendritic cells and certain epithelial cells. This product is routinely tested in flow cytometry on Lewis rat splenocytes.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.4, 0.09% sodium azide, 1% BSA.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.