Rat MHC Class II RT-1B

Mouse

Rat (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

OX-6

Unconjugated

Protein G purified

Unmodified

Rat thymocyte membrane glycoprotein.

Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Does not react with the BDIX rat strain. Expression is polymorphic in mice. This product is routinely tested in flow cytometry on rat splenocytes.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen
• Immunofluorescence
• Flow Cytometry (1:50 - 1:100)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)

PBS, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.