Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone OX-3] (IgG1) to Rat MHC Class II I-Ab/s
IHC - Paraffin, IHC - Frozen, Flow Cytometry
Rat MHC Class II I-Ab/s
Rat, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [OX-3]
IHC - Paraffin
IHC - Frozen
Flow Cytometry (1:100)
Specificity and Use
Rat thymocyte membrane glycoproteins.
Recognizes a polymorphic determinant of rat Ia antigen (RT1Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. MRC OX-3 Reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. This product is routinely tested in flow cytometry on Lewis rat splenocytes.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin embedded sections e. g. trypsin or pronase prior to staining. This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections.
PBS, pH 7.4, 0.09% sodium azide, 1% BSA.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.