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Anti-MHC Class II DQ DR Polymorphic Antibody (clone 49.1, FITC) IHC-plus™ LS-B5329

Note: This antibody replaces LS-C57755

Ordering

Wt. Vol. Conc. Price
50 µg - 0.1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MHC Class II DQ DR Polymorphic Antibodies

Anti-MHC Class II DQ DR Polymorphic Antibody LS-C130767
Mouse Monoclonal (IgG2a) to Sheep MHC Class II DQ DR Polymorphic
Sheep, Human, Bovine, Goat
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Unconjugated
Anti-MHC Class II DQ DR Polymorphic Antibody (clone 49.1, FITC) IHC-plus™ LS-B5329
Mouse Monoclonal [clone 49.1] (IgG2a) to Sheep MHC Class II DQ DR Polymorphic
Sheep, Human, Bovine, Goat
IHC - Paraffin, IHC - Frozen, Immunoprecipitation, Flow Cytometry
FITC Conjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 49.1] (IgG2a) to Sheep MHC Class II DQ DR Polymorphic
Sheep, Human, Bovine, Goat
IHC - Paraffin, IHC - Frozen, Immunoprecipitation, Flow Cytometry (applications tested for the base form of this product only)
FITC Conjugated

Details

Sheep MHC Class II DQ DR Polymorphic
Mouse
Sheep, Human, Bovine, Goat (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [49.1]
Fluorescein. Also available Unconjugated.
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry (1:10)
  • (applications tested for the base form of this product only)

Specificity and Use

Recognizes a polymorphic epitope on ovine MHC class II DQ and DR molecules. In recent work, this clone was found to recognize ovine MHC class II transfectants DQ - T28.1. DR - T8.1. DR - T31.3, but not DQ - T26.2 (Ballingall, K. et al.1995) The expression of ovine MHC class II molecules is restricted to B cells, activated T cells, monocytes, macrophages, dendritic cells and some endothelial and epithelial cells.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1 x 10^6 cells in 100 ul. Method sheets are available on request.

Packaging

PBS, pH 7.4, 0.09% sodium azide, 1% BSA.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MHC Class II DQ DR Polymorphic


Immunohistochemistry

Immunohistochemistry
Anti-MHC Class II DQ DR Polymorphic antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5329 dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-MHC Class II DQ DR Polymorphic antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5329 dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-MHC Class II DQ DR Polymorphic antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5329 dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry
Staining of sheep peripheral blood lymphocytes with MOUSE ANTI OVINE MHC CLASS II DQ/DR (POLYMORPHIC) . This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry
Staining of sheep peripheral blood lymphocytes with MOUSE ANTI OVINE MHC CLASS II DQ/DR (POLYMORPHIC). This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/5/2016

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