Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1D4B] (IgG2a,k) to Mouse MHC Class I H-2Ld/H-2Db
IHC, Immunoprecipitation, Flow Cytometry
Mouse Monoclonal [clone 28-14-8] (IgG2a,k) to Mouse MHC Class I H-2Ld/H-2Db
Mouse MHC Class I H-2Ld/H-2Db
Mouse (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [28-14-8]
Specificity and Use
Mouse MHC Class I
The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2Db, and cross-reacts with H-2Ld, H-2Dq and/or H-2Lq. Binding of 28-14-8 to the alpha 3 domain of H-2Ld is not dependent on ß2-microglobulin.
The 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
Staining of C57Bl/6 splenocytes with 0.25 ug of FITC mouse IgG2a isotype control (open histogram) or 0.25 ug of PE 28-14-8 (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.