(tested or 100% immunogen sequence identity)
MGMT antibody was raised against recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli.
Specific for MGMT (ref 1).
Western Blot: 1:1000~1:3000 (ECL). Immunohistochemistry: 1:2000 (strong [+++] with CEM and no signal [-] with TK6). Positive control: LS174T, Raji, CCRF-CEM,1 Molt-4,1 HeLa CCL2,1 Rh18,1 or HT-292 cells. Liver tissue expresses high levels while brain and bone marrow have low levels of MGMT protein. Use TK6 cells as a negative control.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.