Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Protein A purified
IHC - Paraffin, Western blot (1 - 3 µg/ml), Flow Cytometry (5 - 10 µg/ml)
Specificity and Use
MGMT antibody was raised against recombinant human MGMT protein purified from E. coli.
Recognizes the human MGMT protein. Reactivity is confirmed with human T cell leukemia CEM/CCRF cells.
Suitable for use in Immunohistochemistry, Western Blot and Flow Cytometry. Western Blot: 1-3 ug/ml. Flow Cytometry: 5-10 ug/ml. Immunohistochemistry: 10 ug/ml. Note: Staining results for immunohistochemical assays with formalin-fixed, paraffin-embedded tissue sections may be greatly enhanced with an antigen retrieval pre-treatment step using citrate buffer, pH 6.0.
PBS, pH 7.4, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.