Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 5H2 (2G9/31)] (IgG1) to Human Merosin
Human, Monkey, Rabbit
IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Human, Monkey, Rabbit (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [5H2 (2G9/31)]
IHC - Frozen (1:2000 - 1:10000)
ELISA (1:25000 - 1:100000)
Specificity and Use
Merosin antibody was raised against purified human merosin.
Reacts with the 80 kD fragment of the M-chain of human Laminin alpha2, originally known as Merosin. Laminins are trimeric basement glycoproteins consisting of three non-identical multi-domain chains (alpha, beta and gamma) each encoded by a distinct gene. The alpha2 chain is a subunit of Laminin-2 and Laminin-4, which are widely expressed in the basement membrane of skeletal muscle and peripheral nerves. Laminins are thought to mediate cell attachment, migration, proliferation and differentiation with other extracellular matrix components.
Immunohistology: Acetone is recommended. 8 micron fixed cryostat muscle sections have been used, with an HRP conjugated secondary antibody for detection.
Ascites, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.