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Anti-MER / MERTK Antibody LS-C97474

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MER / MERTK Antibodies

Anti-MER / MERTK Antibody (aa6-37) LS-C99094
Rabbit Polyclonal to Human MER / MERTK
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MER / MERTK Antibody (aa990-999) IHC-plus™ LS-B4445
Goat Polyclonal to Human MER / MERTK
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MER / MERTK Antibody (aa746-757) LS-C72438
Rabbit Polyclonal (IgG) to Human MER / MERTK
Human
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human MER / MERTK
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human MER / MERTK
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:10)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

MER / MERTK antibody was raised against his tag recombinant protein of human MERTK.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About MER / MERTK

Q12866 NM_006343 NP_006334.2

MERTK Antibody, Eyk Antibody, MER Antibody, MER receptor tyrosine kinase Antibody, Nyk Antibody, Proto-oncogene c-Mer Antibody, RP38 Antibody, STK kinase Antibody, C-mer Antibody, Receptor tyrosine kinase MerTK Antibody, Tyrosine-protein kinase Mer Antibody

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human lymphoma reacted with MERTK Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Fluorescent image of U251 cell stained with MERTK Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MERTK primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). MERTK immunoreactivity is localized to Vesicles significantly.

Western blot

Western blot
MER Antibody western blot of Jurkat cell line lysates (35 ug/lane). The MER antibody detected the MER protein (arrow).

Flow Cytometry

Flow Cytometry
Flow cytometric of Jurkat cells using MERTK Antibody(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 12/7/2016

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